TBtools, a Toolkit for Biologists integrating various biologi

Summary Rapid development of high-throughput sequencing (HTS) techniques has led biology into the “big-data” era. Data analysis using various bioinformatics softwares or pipelines relying on programming and command-line environment is challenging and time-consuming for most wet-lab biologists. Bioinformatics tools with a user-friendly interface are preferred. Here, we present TBtools (a Toolkit for Biologists integrating various biological data handling tools), a stand-alone software with a user-friendly interface. It has powerful data handling engines for both bulk sequence processing and interactive data visualization. It includes a large collection of functions, which may facilitate much simple, routine but elaborate work on biological data, such as bulk sequence extraction, gene set enrichment analysis, Venn diagram preparation, heatmap illustration, comparative sequence visualization, etc.